Experimental approach
Pregnant rats were administrated with 11β-HSD2 inhibitor carbenoxolone
(CBX) subcutaneously or by placenta-targeted delivery system. The blood
pressure, renal and placental morphology, placental blood flow and
circulatory levels of fms-like
tyrosine kinase 1 (sFlt1) and placental growth factor (PlGF) were
subsequently examined. Cultured human trophoblasts were used to
investigate the role of 11β-HSD2 in migration and invasion function and
sFlt1 release in vitro .