Immunofluorescent analysis
Paraffin sections (5μm) of placentas were rehydrated and incubated in
sodium citrate buffer (pH 6.0) for 10min to retrieve antigens. After
blocking in 3% H2O2, the slides were
incubated with antibody against laminin (1:200; Abcam, Cat# ab11575;
RRID: AB_298179) at 4°C overnight. After washing 3 times with PBS, the
sections were incubated with Alexa fluor 488 donkey anti-mouse IgG(H+L)
(1:1,000 dilution) for 2h in dark at room temperature. Then the slides
were mounted with cover glass by mounting solution, and the picture was
taken under Zeiss LSM 780 confocal microscope (Carl Zeiss Inc., Jena,
Germany).