FIGURE LEGENDS
Figure 1. Regulation of PD-L1 mRNA level in blood cells
of pre-school children. (a) Experimental design of the blood and
nasopharyngeal fluid (NPF) analysis of the healthy (n=21) and asthmatic
(n=24) pre-school children of the PreDicta cohort in Erlangen.(b) PD-L1/HPRT mRNA expression in total blood cells of
healthy and asthmatic children with a virus-induced (v) asthma phenotype
at the baseline visit (n=10/11). (c) PD-L1/HPRT mRNA
expression in total blood cells of healthy and asthmatic children
(n=10/17). (d) PD-L1/HPRT mRNA expression in total blood
cells of healthy and asthmatic children subdivided according to their
FEV1% at the baseline visit (n=0/2/9/15). (e) Correlation of
the PD-L1/HPRT mRNA level in total blood cells of asthmatic
children with the FEV1% at the baseline visit. (f)PD-L1/HPRT mRNA expression in total blood cells of healthy and
asthmatic children subdivided according to their PEF% at the baseline
visit (n=5/6/5/10). (g)Correlation of the PD-L1/HPRT mRNA level in total blood cells of
asthmatic children with the PEF% at the baseline visit. (h)Correlation of the PD-L1/HPRT mRNA level in total blood cells of
asthmatic children with rhinovirus in their airways with the FEV1%
(top) and PEF% (bottom) at the baseline visit. Data are presented as
means ± SEMs. Two-tailed student t test (b, c) or ordinary
one-way ANOVA (d, f) was used to calculate statistical significance. * p
≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.001, **** p≤ 0.0001.
Figure 2. IFNβ correlated with better lung function in
asthmatic children
(a, b) Correlation of the IFNβ level, measured in the
supernatants of the untreated PBMC culture and the respective FEV1% (i)
and PEF% (j) of asthmatic children with and without rhinovirus in their
airways. Data are presented as means ± SEMs. Two-tailed student ttest (b, c) or ordinary one-way ANOVA (d, f) was used to calculate
statistical significance. * p ≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.001, ****
p≤ 0.0001.
Figure 3. Correlation between PD-L1 mRNA level with IFNβ
in healthy and asthmatic patients. Correlation of the PD-L1/HPRTmRNA level in total blood cells with the IFNβ level, measured in the
supernatants of the respective untreated and with rhinovirus 1b
re-stimulated PBMC culture, of healthy and asthmatic children.
Figure 4. PD-L1 is upregulated in blood cells of asthmatic
children with high C-reactive protein (CRP) serum levels and correlated
with RV in the
airways
(a) PD-L1/HPRT mRNA expression in total blood cells of
healthy and asthmatic children subdivided according to their CRP serum
level (n=9/10/2). (b,c) Correlation of the PD-L1/HPRTmRNA level in total blood cells with the CRP serum level subdivided in
healthy and asthmatic children with and without rhinovirus in their
airways. Data are presented as means ± SEMs. Ordinary one-way ANOVA was
used to calculate statistical significance. * p ≤ 0.05; ** p ≤ 0.01, ***
p ≤ 0.001, **** p≤ 0.0001.