Figure 6: Vit D3 rescued CD4+ T cells inhibited PD1+CD4+ T cells in the absence of ST2 after RV infection of lung cells
(a) Experimental design. WT B6 n=4. ST2-/- n=2. IL33-/- n=4. (b) Analysis of CD4+ lymphocytes from wild-type and ST2-/- mice, infected with RV1b and afterwards treated with 1a,25 DihodroxyvitaminD3 (=VitD3) and/or OVA in vitro. p=0,029. p=0,009. p=0,026. Two-tailed Student’s T test. p= 0,00028. p=0,022. p=0,028. Two-tailed Student’s T test.
(c) PD1+CD4+ cells gated on CD4+lymphocytes, from wild-type (n=4) and ST2-/- (n=2) mice, infected with RV1b and afterwards treated with 1a,25 DihodroxyvitaminD3 (=VitD3) and/or OVA in vitro. p=0,009. p=0,048. p=0,045. Two-tailed Student’s T test.