Figure legend
Figure 1. Impact of HIV infection and ART on frequencies of CD4+
T cell subsets. Flow cytometry analyses of PBMCs in HIV-infected and
healthy subjects.(A) Gating strategy to identify CD4+ T cell subsets(TN,
TCM, TEM, TemRA).(B) The frequencies of CD4+ T cell subsets in
HIV-infected and healthy subjects, including TN(a), TCM(b), TEM(c),
TemRA(d). Measurements were performed on samples from 30 ART naive
patients, 30 ART patients and 28 healthy subjects. About 5 PBMC samples
were analyzed fresh within a working day and a total of 18 independent
experiments were performed. Median [IQR] values are indicated.
Significant differences were determined in a non-parametric
Kruskal-Wallis test: *p < 0.05, **p <
0.01, ***p < 0.001.
Figure 2. Impact of HIV infection and ART on replicative
senescence(CD57+)and activation
(HLADR+) expression within CD4 T-cell subsets (see
Fig.1) .
Flow cytometry analyses of PBMCs in HIV-infected and healthy subjects.
(A)Left: Representative dot plots showing the proportion of CD4
subsets-expressing CD57+ T cells. Right:
Representative dot plots showing the proportion of CD4
subsets-expressing HLADR+T cells.(B)Percentage of CD4
subsets-expressing CD57+ and HLADR+T cells in HIV-infected and healthy subjects: including TN(a-b),
TCM(c-d), TEM(e-f), TemRA(g-h). Measurements were performed on samples
from 30 ART naive patients, 30 ART patients and 28 healthy subjects.
About 5 PBMC samples were analyzed fresh within a working day and a
total of 18 independent experiments were performed. Median [IQR]
values are indicated. Significant differences were determined in a
non-parametric Kruskal-Wallis test: *p < 0.05,
**p < 0.01, ***p < 0.001.
Figure 3. Impact of HIV infection and ART on telomere length of
naive and memory T cells in HIV-infected and healthy subjects .
Telomere length values of naive T cells(Left) and memory T
cells(Right)were assessed. Measurements were performed on samples from
21 ART naive patients, 18 ART patients and 24 healthy subjects. Data are
shown as mean±SD and are pooled from four independent experiments and
the number of samples in each experiment is 24,16,16,7, respectively.
*p < 0.05, **p < 0.01, ***p< 0.001; one-way analysis of variance .