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Figure 1. Impact of HIV infection and ART on frequencies of CD4+ T cell subsets. Flow cytometry analyses of PBMCs in HIV-infected and healthy subjects.(A) Gating strategy to identify CD4+ T cell subsets(TN, TCM, TEM, TemRA).(B) The frequencies of CD4+ T cell subsets in HIV-infected and healthy subjects, including TN(a), TCM(b), TEM(c), TemRA(d). Measurements were performed on samples from 30 ART naive patients, 30 ART patients and 28 healthy subjects. About 5 PBMC samples were analyzed fresh within a working day and a total of 18 independent experiments were performed. Median [IQR] values are indicated. Significant differences were determined in a non-parametric Kruskal-Wallis test: *p < 0.05, **p < 0.01, ***p < 0.001.
Figure 2. Impact of HIV infection and ART on replicative senescence(CD57+)and activation (HLADR+) expression within CD4 T-cell subsets (see Fig.1) .
Flow cytometry analyses of PBMCs in HIV-infected and healthy subjects. (A)Left: Representative dot plots showing the proportion of CD4 subsets-expressing CD57+ T cells. Right: Representative dot plots showing the proportion of CD4 subsets-expressing HLADR+T cells.(B)Percentage of CD4 subsets-expressing CD57+ and HLADR+T cells in HIV-infected and healthy subjects: including TN(a-b), TCM(c-d), TEM(e-f), TemRA(g-h). Measurements were performed on samples from 30 ART naive patients, 30 ART patients and 28 healthy subjects. About 5 PBMC samples were analyzed fresh within a working day and a total of 18 independent experiments were performed. Median [IQR] values are indicated. Significant differences were determined in a non-parametric Kruskal-Wallis test: *p < 0.05, **p < 0.01, ***p < 0.001.
Figure 3. Impact of HIV infection and ART on telomere length of naive and memory T cells in HIV-infected and healthy subjects .
Telomere length values of naive T cells(Left) and memory T cells(Right)were assessed. Measurements were performed on samples from 21 ART naive patients, 18 ART patients and 24 healthy subjects. Data are shown as mean±SD and are pooled from four independent experiments and the number of samples in each experiment is 24,16,16,7, respectively. *p < 0.05, **p < 0.01, ***p< 0.001; one-way analysis of variance .