3.6 Effects of NAC on SNP responses are mediated by formation of H2S
In vitro , in blood samples from patients with chronic CAS, NAC induced concentration-dependent potentiation of the anti-aggregatory effects of SNP. Similar effects were more potently induced by the direct H2 S donor NaHS (p < 0.01 for both) (Fig. 6 ).
We then assessed the potential impact of inhibitors of H2S formation [PAG (3.3mM) and AOAA (0.5mM)] on NAC-induced potentiation of the anti-aggregatory effects of SNP . There was a significant diminution of SNP effect, both with PAG (p = 0.0002) and with AOAA (p = 0.001: Fig. 7 )