3.6 Effects of NAC on SNP responses are mediated by formation of
H2S
In vitro , in blood samples from patients with chronic CAS, NAC
induced concentration-dependent potentiation of the anti-aggregatory
effects of SNP. Similar effects were more potently induced by the direct
H2 S donor NaHS (p < 0.01 for both)
(Fig. 6 ).
We then assessed the potential impact of inhibitors of
H2S formation [PAG (3.3mM) and AOAA (0.5mM)] on
NAC-induced potentiation of the anti-aggregatory effects of SNP . There
was a significant diminution of SNP effect, both with PAG (p = 0.0002)
and with AOAA (p = 0.001: Fig. 7 )