loading page

Comprehensive RNA-seq Analysis Revealed Molecular Pathways and Genes Associated with Drought Tolerance in Wild Soybean (Glycine soja Sieb. & Zucc.)
  • +4
  • Muqadas Aleem,
  • Muhammad Raza,
  • Muhammad Haider,
  • Rana Atif,
  • Zulfiqar Ali,
  • Javaid Bhat,
  • Tuanjie Zhao
Muqadas Aleem
Nanjing Agricultural University College of Agriculture
Author Profile
Muhammad Raza
Nanjing Agricultural University College of Agriculture
Author Profile
Muhammad Haider
Nanjing Agricultural University
Author Profile
Rana Atif
University of Agriculture Faisalabad
Author Profile
Zulfiqar Ali
MNS-University of Agriculture
Author Profile
Javaid Bhat
Nanjing Agricultural University
Author Profile
Tuanjie Zhao
Nanjing Agricultural University
Author Profile

Abstract

Drought stress at germination stage is an important environmental stress limiting crop yield. Hence, our study investigated comparative root transcriptome profiles of four contrasting soybean genotypes viz., drought-tolerant (PI342618B/DTP & A214/DTL) and drought-sensitive (NN86-4/DSP & A195/DSL) under drought stress using RNA-Seq approach. Total of 4850 and 6272 differentially expressed genes (DEGs) were identified in tolerant (DTP & DTL) and sensitive (DSP & DSL) genotypes, respectively. Principle component analysis (PCA) and correlation analysis revealed higher correlation of DTP with DTL. Both gene ontology (GO) and MapMan analyses showed drought response was enriched in the DEGs associated with water and auxin transport, cell wall/membrane, antioxidant activity, catalytic activity, secondary metabolism, signaling and transcription factor (TF) activities. Out of 981 DEGs screened from above terms, only 547 showed consistent opposite expression between contrasting genotypes. Twenty-eight DEGs of 547 were located on Chr.08 rich in QTLs and “Hotspot regions” associated with drought stress, and eight of them showed non-synonymous SNP polymorphism. Hence, ten genes (including above eight genes plus two hub genes) were predicated as possible candidates regulating drought tolerance, which needs further functional validation. Overall, the transcriptome profiling provided in-depth understanding about the genetic mechanism and candidate genes underlying drought tolerance in soybean.