qRT-PCR Validation
In order to validate the reliability and reproducibility of RNA-seq
results, we selected above ten predicated candidate genes plus three
random DEGs related to stress response for qRT-PCR validation
(Table S1 ). By comparing qRT-PCR and RNAseq data, the relative
expression of qRT-PCR analysis was also converted into FC by base 2 for
consistency with the RNA-Seq data. These 12 genes include both
up-regulated and down-regulated genes (Table S14 ). The results
of qRT-PCR analysis of all these 12 genes showed consistency with the
RNAseq data (Fig. 10). Overall, the results of qRT-PCR analysis
showed the same expression trends as that of RNA-Seq, indicating the
reliability of transcriptomic data used for the analysis in the present
study.