Total and annotated number of ZOTUs
A total of 2,980 ZOTU sequences were created using Usearch. The mean ZOTU sequences per sample were 961. Among 12 samples Site 5-1 was the most abundant sample with 1,176 ZOTU sequences, while Site 3-1 was the least abundant sample with 725 ZOTU sequences (Table 1).
Among all ZOTU sequences, 1,495 ZOTU sequences (50.17%) could be annotated to genera or lower taxonomy level. The mean number of identified taxa per sample is 542. Among the 12 samples Site 2-1 was the most abundant sample with 762 known taxa, while Site 4-1 was the least abundant sample with 274 known taxa (Table 1). Of the 1,495 identifiable ZOTU sequences, 1243 ZOTU sequences were identified to 134 species of diatoms and 221 ZOTU sequences were identified to 77 genera of diatoms (Fig S2). Amphora , Aulacoseira , Diadesmis ,Gomphonema , Lemnicola , Melosira , Placoneis ,Planothidium , Sellaphora , and Stauroneis are the dominant genera in the 12 samples, and Diadesmis confervacea ,Gomphonema parvulum , Lemnicola hungarica , Melosira varians , Placoneis elginensis , Sellaphora pupula , andStauroneis kriegeri are the dominant diatom species.