Tables
Table 1. Soil samples collected from suspect’s pants, crime scene and
nearby areas.
Table 2. Primers for amplifying diatom rbcL fragments together
with oligoes attached to the 5’ ends of the primers for labeling 12 soil
samples.
Figures
Fig. 1. Localities of soil samples collected as references for tracking
the possible source of the mud on the suspect’s pants and pictures of
crime scene, mud from the canal, and suspect’s pants. A: Google map of
five sampling sites; B: close-up of Site 1 to 3; C: the crime scene, D:
soil from the canal; and E: suspect’s pants.
Fig. 2. Diagram showing how rbcL fragments were labeled by PCR,
pooled together and sequenced on Ion Torrent S5xl platform.
Fig. 3. Percentages of the ZOTUs in the mud on suspect’s pants tracked
to the 11 candidate source sites by FEAST. A: total ZOTUs with
singletons; B: total ZOTUs without singletons; C: annotated ZOTUs with
singletons; and D: annotated ZOTUs without singletons.
Fig. S1. Experiment design. Five parts, soil DNA acquisition, amplicon
preparation, NGS data sequencing, NGS data processing, and suspect’s mud
tracking.
Fig. S2. The ZOTU-rich diatom genera (A) and species (B) found in 12
soil samples.
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