Phylogenetic relationship of South Tyrolean M. myotis with
European individuals based on mitochondrial haplotypes (HVII)
To investigate the phylogenetic relationship between South TyroleanM. myotis and individuals from neighboring areas we used
sequences only from the HVII region of the mitochondrion, which has been
widely used throughout Europe (Bogdanowicz, LesiĆski, Sadkowska-Todys,
Gajewska, & Rutkowski, 2013; Bryja et al., 2010; Castella et al., 2001;
Furman et al., 2014; Ruedi & Castella, 2003; Ruedi et al., 2008).
Sequences from this study were trimmed to include the sole HVII region
and were aligned with representative sequences of different haplotypes
reported in Europe for this bat species using Mafft with the G-INS-I
setting (Katoh, Misawa, Kuma, & Miyata, 2002) (Table S3). Phylogenetic
relationship between individuals was determined using ML tree
implemented in PhyML 3.0 software using the general time-reversible
(GTR) model of nucleotide substitution with gamma-distributed rate
variation among sites (with four rate categories, G4), a heuristic SPR
branch-swapping search and 1000 bootstrap replications to assess the
robustness of individual nodes (Dereeper et al., 2008). To obtain the
best representation of the haplotype diversity in our study area,
phylogenetic analyses were run using all available samples, thus
including adult males and youngsters in their first year of age captured
during the reproductive season, either in May or September (Table 1).