Phylogenetic relationship of South Tyrolean M. myotis with European individuals based on mitochondrial haplotypes (HVII)
To investigate the phylogenetic relationship between South TyroleanM. myotis and individuals from neighboring areas we used sequences only from the HVII region of the mitochondrion, which has been widely used throughout Europe (Bogdanowicz, LesiƄski, Sadkowska-Todys, Gajewska, & Rutkowski, 2013; Bryja et al., 2010; Castella et al., 2001; Furman et al., 2014; Ruedi & Castella, 2003; Ruedi et al., 2008). Sequences from this study were trimmed to include the sole HVII region and were aligned with representative sequences of different haplotypes reported in Europe for this bat species using Mafft with the G-INS-I setting (Katoh, Misawa, Kuma, & Miyata, 2002) (Table S3). Phylogenetic relationship between individuals was determined using ML tree implemented in PhyML 3.0 software using the general time-reversible (GTR) model of nucleotide substitution with gamma-distributed rate variation among sites (with four rate categories, G4), a heuristic SPR branch-swapping search and 1000 bootstrap replications to assess the robustness of individual nodes (Dereeper et al., 2008). To obtain the best representation of the haplotype diversity in our study area, phylogenetic analyses were run using all available samples, thus including adult males and youngsters in their first year of age captured during the reproductive season, either in May or September (Table 1).