Suppl. Figure 1.
The Hh GRN. Note the negative feed-back loop between SMOsignaling and PTCH 1/2. While low dose SHH would be expected to lower PTCH1/2 on the surface of responding cells, the knock-down of PTCH1/2 via MO follows a distinct time course. Inptch K/D experiments the optimal SMO dose is reduced by over half compared with low dose SHH/SMO co-injection. Hence, the changes in morphology and gene expression are not necessarily the same.