Suppl. Figure 2
The results of SMO mRNA dose-response pilot experiments for our over-expression bioassay development. Abnormal phenotypes are not consistently observed with single injections of low dose of SHHor even a high dose of SMO . On the other hand, a 2pg ofSHH and 200pg of SMO mRNA (co-injection) significantly induced abnormal phenotypes compared with all other dose conditions [except 1pg of SHH and 600pg of SMO ]. For this pilot study, the injections for 50 embryos on each group were performed in triplicate and on different days. Error bars represent means with SD. **P < 0.01 (Student’s t-test).