Figure 4.
Functional analysis of representative domain variants were conducted using low dose MO of ptch1 and ptch2 . WT zebrafish embryos were injected with low dose ptch1 and ptch2 MO as well as with each of the four distinct SMO mRNA transcripts and harvested at 24hpf. (A ) The eye area was measured at 24hpf. Significant differences in eye area observed in SMO p.V404M when compared togfp , SMO p.R113Q, p.R199W and p.P687L. Representative eye images of gfp (a) and SMO p.V404M (b) are shown upper panels. (B ) Somite angle was measured at 24hpf. Significant difference in somite angle observed in SMO p.R113Q when compared to gfp , SMO p.R199W and p.P687L. Representative somite images of gfp (a’) and SMO p.R113Q (b’) are shown upper panels. Error bars represent means with SD. *P < 0.05 (Student’s t-test).