trnL-trnF region
Plastid data obtained from trnL-trnF sequences shown in Figure 2A
(NJ), 2B (MP) and 2C (ML) resemble those found in the ITS sequences
analysis, but in general with lower resolution. The trees obtained from
analysed sequences are divided into two major clades too. The first
clade consists of members of the subgenus Eriostemones (T.
sylvestris including both subspecies), which is moderately
supported by NJ (86%) and ML (74%) but not supported by MP
(<50%). In the Eriostemones clade, the
published sequence of T. sylvestris subsp. sylvestrisshowed sequence differences to the newly obtained sequences of the
species. The second clade consists of members of the subgenusTulipa, belonging to section Tulipa (T. albanica, T.
kosovarica, T. luanica, T. scardica, T. serbica, T. suaveolens andT. gesneriana ), section Tulipanum (T. julia andT. ulophylla ) and section Spiranthera (T.
tschimganica ). The subgenus Tulipa is moderately
supported by NJ (87%), MP (89%) and ML (89%). Furthermore, the
specimens of subgenus Tulipa divides into two subclades, the
first subclade consists of T. kosovarica, T. Luanica and T.
serbica, which was moderately supported by NJ (86%) but not supported
by MP and ML, while the second subclade consisting of T. albanica,
T. scardica, T. julia, T. ulophylla, T. Suaveolens and T.
tschimganica was moderately supported by NJ (84%) and ML (76%), but
not supported by MP. Within the first subclade, in all phylogenetic
trees T. kosovarika (samples T6, T7 and T8) were further divided
with weak support by NJ (63%), ML (62%) and MP (67%).
Figure 2. Phylogenetic tree constructed from trnL-trnFdataset of the Tulipa taxa.