Measurement of cytokines by Th1/Th2 Cytokine 11-Plex Human ProcartaPlex™ assay
Cytokines were measured in serum collected from whole blood. Also, in cellular supernatants of PBMC monolayers after 18 h of incubation with and without PPD antigen stimulation. Concentrations of IFN-γ, IL-12, IL-2, TNF-α, GM-CSF, IL-1, IL-13, IL-4, IL-5, IL-6 and IL-18 were measured in cell supernatants with a Th1/Th2 Cytokine 11-Plex Human ProcartaPlex™ assay from ThermoFischer Scientific, USA. Readings were taken on a Luminex 100 instrument (Luminex technologies, USA).