Materials and Methods
We studied peripheral blood cells from health endemic controls (EC),
LTBi cases, diabetics with and without LTBi and TB patients.
Mycobacterial antigen-stimulated cytokine secretion was determined using
the Th1/Th2 11 plex cytokine assay. Antigen-induced gene expression of
IFNγ, TNFα, IL6 and SOCS3 was determined by reverse-transcription PCR.