3.4. FAS assay using partially purified FAS I and II
The Assays using partially purified enzymes were performed to verify the
FAS I and FAS II inhibitory activities of Phormidin (Figure 4a. and b.).
Phormidin showed comparable results with Cerulenin and C75 at lower
concentration of the title compound and higher inhibition at higher
concentration of Phormidin. The graphs (Figures 5a. and b.) were plotted
with change in concentration of NADP to NADPH against time. Each point
in graph was the concentration of NADPH measured at 340 nm (Table 2).