Figure 1 Effects of biuret on dry weight (a, d), biuret concentration (b, e), and allantoin concentration (c, f) in roots and shoots of 7-day-old wild-type rice plants (a–c) and 9-day-oldbiuret hydrolase overexpressing rice lines (d–f). Bars and circles represent mean and each sample, respectively. nd means not detected. (a–c) Wild-type plants were grown with 0, 0,1, and 0.3 mmol L-1 biuret supplemented in the culture solution. Ten seedlings were combined for a single sample. Different alphabets indicate significant difference among treatments in each organ (p< 0.05, Tukey’s test, n = 3). (d–f) Wild-type and two independent transgenic lines (B3-9-1 and B2-3-3) were grown with or without 0.3 mmol L-1 biuret in the culture solution. Four to six plants were combined into one sample. Gray and black bars represent control and biuret-treated plants, respectively. Asterisks indicate significant difference between the treatment (*p< 0.05; **p < 0.01, Welch’s t-test, n = 3).