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Three new CYP450 from Betula platyphylla Suk. with 28 oxidation function catalyze the conversion of lupineol to betulinic acid
  • +5
  • jie yang,
  • ying li,
  • yuqi zhang,
  • lu sun,
  • siyao wang,
  • jialei xiao,
  • yaguang zhan,
  • jing Yin
jie yang
Northeast Forestry University
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ying li
Northeast Forestry University
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yuqi zhang
Northeast Forestry University
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lu sun
Northeast Forestry University
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siyao wang
Northeast Forestry University
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jialei xiao
Northeast Agricultural University
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yaguang zhan
Northeast Forestry University
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jing Yin
Northeast Forestry University
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Peer review status:UNDER REVIEW

05 Apr 2020Submitted to Plant, Cell & Environment
08 Apr 2020Assigned to Editor
08 Apr 2020Submission Checks Completed
10 Apr 2020Reviewer(s) Assigned
29 Apr 2020Review(s) Completed, Editorial Evaluation Pending

Abstract

Birch(Betula platyphylla Suk.) bark contains important pentacyclic triterpenes as betulin and betulinic acid, which play important functions in anti-tumor and anti-HIV.Cytochrome P450 monooxygenase(CYP450) is essential for the diversification and functional modification of the triterpene skeleton.In this study, five new CYP450 genes were cloned from birch with ORF lengths of 1284bp, 1533bp, 1188bp, 1704bp, and 1539bp, respectively.Phylogenetic tree analysis shown that five BpCYP450 genes are located in five subfamilies, named CYP94B89, CYP89S1, CYP97B62, CYP86B54, and CYP86A182.The expression characteristics of five CYP450 genes in different tissues and their responses to different stresses(MeJA, SA, GA3, ABA, ethylene, and mechanical damage) were significantly different, among which CYP89S1, CYP97B62 and squalene epoxidase(BpSE) and dammarenediol synthase(BpDS) were highly expressed in leaves.CYP89S1, CYP97B62, and CYP86A182 genes are induced by MeJA and significant synergistic expression effects with lupeol synthase(BpW).CYP89S1, CYP97B62, CYP86A182 have C-28 oxidation function and catalyzing the conversion of lupeol to betulinic acid.Among them, CYP97B62 gene has the highest catalytic efficiency, increasing the content of betulinic acid by 1136%.In addition, co expression of BpMYB21 and CYP86A182 can significantly enhance the conversion and synthesis efficiency of betulinic acid in tobacco(Nicotiana tabacum L.), and CYP89S1 can enhance salt and alkali resistance in yeast(Saccharomyces cerevisiae)