AMH assay:
Anti-Mullerian hormone was analyzed by “Beckman Coulter Access II” enzymatic-immunoassay. In the assay, calibrators, controls and samples are incubated in microtitration wells which have been coated with anti-AMH antibody. After serial enzymatic reactions and washing steps, the degree of enzymatic turnover of the substrate is determined by dual wavelength absorbance measurement at 450 nm and between 600 and 630 nm. The absorbance measured is directly proportional to the concentration of AMH in the samples. A set of AMH calibrators is used to plot a calibration curve of absorbance versus AMH concentration. The AMH concentrations in the samples Then can be calculated from this calibration curve.