Emergence of A Novel Disease Caused by Staphylococcus Pseudintermedius
in Raccoon Dogs (Nyctereutes procyonoides)
Abstract
This study reports outbreak of a new disease caused by Staphylococcus
Pseudintermedius in Raccoon dogs. The disease occurred in a breeding
farm of raccoon dogs in Guan County of Shandong Province in China in
August of 2019. 47% (425/896) of raccoon dogs showed the abnormal
symptoms; 17.6% (75/425) of them had sever skin and soft tissue
infections(SSTIs), dyspnea and sever pathological lesions in lungs,
hearts, livers, kidneys, etc; 4.2% (18/425) were dead in 4 weeks. The
pathogen of the disease was identified as Staphylococcus
pseudintermedius by mass spectrometer detection, microscopic examination
and biochemical reaction test. Its nucleotide homology of 16SrDNA was
100% with thats of other published strains and its genotype was between
American strain and Brazilian strain from other animal species. The
isolated Staphylococcus pseudointermediate strain from the diseased
raccoon dogs could cause ulceration and suppuration in the skins and
sever pathological lesions in some organs of the infected mice, and it
is confirmed as a methicillin-resistant Staphylococcus pseudintermedius
(MRSP) strain by the amplification of mecA gene; and 12 sensitive drugs
were screened by drug sensitivity test. Full attention should be paid to
the great economic loss and the potential zoonotic risk caused by the
Staphylococcus pseudintermedius in raccoon dogs, and this study can
provide a reference for the clinical diagnosis and treatment of this new
disease.