Pathogenicity of the isolated S. pseudintermedius in mice
To assess the pathogenicity of the isolated S. pseudintermedius,6 mice were artificially inoculated using the purified bacterial. After 14 days post inoculation (dpi), the symptoms such as lethargy, depression, loss of appetite, closing eyes, fear of cold, drowsiness, crouching in cages, fur wrinkles occurred in all the six mice; in five of six mice, the obvious ulceration and suppuration appeared in the abdominal skins or hind limb (Fig. 7); but none of six mice was dead in two weeks. After anatomy, swelling and bleeding in varying degrees occurred in the lungs, hearts, livers, kidneys; even hemorrhage and necrosis did in some mice (Fig. 8), which were consistent with those of the diseased raccoon dogs. Correspondingly, the pathogen was isolated from the ulcerative skin and lungs of the infected mice, and was identified as S. pseudintermedius . It was confirmed that the isolated S.pseudointermediate from the diseased raccoon dogs can cause sever lesions in mice.