Pathogenicity caused by the isolated pathogenic bacterial in mice
The isolated pathogenic bacterial strain was inoculated into TSB medium and cultured until the logarithmic growth phase at 37 ℃. The colony-forming units (CFU) of the bacterial was calculated by 10-fold dilution according to the published method (Savini et al., 2013; Ma et al., 2018a). Six BALb/c mice from S1 to S6 were intraperitoneally inoculated with 1×109 CFU/mL of bacterial solution, each of which was 0.2mL. Control mice from C1 to C2 were intraperitoneally inoculated with sterilized TSB medium, each with 0.2 mL. After inoculation, the mice were observed for describing their clinical symptoms for two weeks and then were dissected for assessing the pathological lesions in some internal organs. Finally, the pathogenic bacterial in the inoculated mice was isolated and identified according to the previous referred methods.