Drug sensitivity test
According to the Kirby-Bauer disk method designated by Clinical and
Laboratory Standards Institute (CLSI), 24 antimicrobial agents were used
for drug sensitivity test. The specific operations were as follows: the
isolated bacterial strains were picked up and cultured in TSB medium at
37 ℃for 18h-20h. The 0.2 mL bacteria suspension was taken on TSA medium
and smeared evenly with a coating rod. After standing for a few minutes,
the drug sensitive tablets were spread equidistantly on the surface of
the plate with sterile tweezers and cultured at 37 ℃ for 24h. The
diameter of bacteriostatic zone was measured and its sensitivity was
determined according to CLSI standard.