Mass spectrometer detection
Three diseased raccoon dogs’ tissues including lung, heart, liver, kidney, pus and lymph node were collected aseptically. Each tissue sample was inoculated into TSA culture medium and cultured for 18-24h at 37℃. The single colony was picked up to smear on the MALDI target plate and detected using a automatic rapid biomass spectrum detection system (IVD MALDI Biotyper, Bruker Daltonic Inc, U.S.A.) according to the instructions, and then the detected results of the samples were compared with the microbial protein fingerprint in the database and the pathogenic microbes were statistically analyzed.