Amplification of mecA gene and screening of sensitive drugs
To analyze the resistance of the isolated bacteria and screen the sensitive drugs against it, the resistant mecA gene was amplified and drug-sensitivity test was conducted. The results in Fig.9 showed that the mecA gene with the size of 310bp could be detected from the isolated strains using PCR, which suggests that the isolated strain is methicillin-resistant. The results in Table 3 showed that the isolated S.pseudintermedius strain is sensitive to twelve drugs including enrofloxacin, ofloxacin, neomycin, chloramphenicol, meropenem, and amikacin, ciprofloxacin, polymyxin B, streptomycin, cephalothin, florfenicol, erythromycin; but resistant to nine drugs including kanamycin, gentamicin, lincomycin, compound sulfamethoxazole, sulfamethoxazole, tetracycline, aztreonam, penicillin G and vancomycin; especially, completely resistance to lincomycin, compound sulfamethoxazole and aztreonam.