Amplification of mecA gene and screening of sensitive
drugs
To analyze the resistance of the isolated bacteria and screen the
sensitive drugs against it, the resistant mecA gene was amplified
and drug-sensitivity test was conducted. The results in Fig.9 showed
that the mecA gene with the size of 310bp could be detected from
the isolated strains using PCR, which suggests that the isolated strain
is methicillin-resistant. The results in Table 3 showed that the
isolated S.pseudintermedius strain is sensitive to twelve drugs
including enrofloxacin, ofloxacin, neomycin, chloramphenicol, meropenem,
and amikacin, ciprofloxacin, polymyxin B, streptomycin, cephalothin,
florfenicol, erythromycin; but resistant to nine drugs including
kanamycin, gentamicin, lincomycin, compound sulfamethoxazole,
sulfamethoxazole, tetracycline, aztreonam, penicillin G and vancomycin;
especially, completely resistance to lincomycin, compound
sulfamethoxazole and aztreonam.