Genotype of the isolated S. pseudintermedius determined by 16SrDNA sequence analysis
To determine the genotype of the isolated S. pseudintermedius , 16SrDNA gene was amplified from the strain samples and its homology and phylogenetic tree were analyzed using DNA Star software. The results in Fig.4 showed that 16SrDNA gene with the size of 1465 bp was successfully amplified, and its nucleotide homology with that of other nine published strains was 100% (Fig.5); and 16SrDNA gene of the isolated strain was named as Sp-SDAU . The phylogenetic analysis showed thatSp-SDAU strain was between American strain (GenBank:FJ536213) and Brazilian strain (GenBank:KX242544) from other animals (Fig. 6).