Pathogenicity caused by the isolated pathogenic bacterial in
mice
The isolated pathogenic bacterial strain was inoculated into TSB medium
and cultured until the logarithmic growth phase at 37 ℃. The
colony-forming units (CFU) of the bacterial was calculated by 10-fold
dilution according to the published method (Savini et al., 2013; Ma et
al., 2018a). Six BALb/c mice from S1 to S6 were intraperitoneally
inoculated with 1×109 CFU/mL of bacterial solution,
each of which was 0.2mL. Control mice from C1 to C2 were
intraperitoneally inoculated with sterilized TSB medium, each with 0.2
mL. After inoculation, the mice were observed for describing their
clinical symptoms for two weeks and then were dissected for assessing
the pathological lesions in some internal organs. Finally, the
pathogenic bacterial in the inoculated mice was isolated and identified
according to the previous referred methods.