Genotype of the isolated S. pseudintermedius determined
by 16SrDNA sequence analysis
To determine the genotype of the isolated S. pseudintermedius ,
16SrDNA gene was amplified from the strain samples and its homology and
phylogenetic tree were analyzed using DNA Star software. The results in
Fig.4 showed that 16SrDNA gene with the size of 1465 bp was successfully
amplified, and its nucleotide homology with that of other nine published
strains was 100% (Fig.5); and 16SrDNA gene of the isolated strain was
named as Sp-SDAU . The phylogenetic analysis showed thatSp-SDAU strain was between American strain (GenBank:FJ536213) and
Brazilian strain (GenBank:KX242544) from other animals (Fig. 6).