The pathogen of the disease was identified as S. pseudintermedius by mass spectrometery
To obtain the pathogenic microbes, the samples from six tissues of the diseased raccoon dogs were inoculated onto the specific TSA medium in Petri Dishes and the cultured bacterial strains were detected using a mass spectrometer. The results in Table 1 showed that 8 species of bacteria were identified from the pus samples of the suppurative focuses, 5 species from Lymph nodes, 4 species from the lungs and liver, 2 species from the kidney. Among these isolated bacteria strains, two strains including staphylococcus pseudintermedius (S. pseudintermedius) and enterococcus faecalis (E.F) were common and the isolated positive ratios of S. pseudintermedius in all the tissues were the highest except in pus. So it was deduced that the pathogen of the disease in raccoon dogs might be S. pseudintermedius .