No. CT, threshold cycle values undetectable using real-time PCR.
No. Tp, time-of-positivity values undetectable using LAMP.
†, In duplicate analyses, one sample was positive, and one was negative using the LAMP reaction.
Fig. 1. Concept of the development of an on-site diagnostic test using a combination of simple DNA extraction with EZ-Fast and a portable battery-powered LAMP device, potentially applicable to a portable real-time PCR device.