Table 3. The effect of polymerase choice. Total number of detected sequence clusters in root nodule samples (Field-Samples-2) amplified using Phusion (proofreading) or Platinum (non-proofreading) polymerases. Sequences were clustered using MAUI-seq, DADA2, and UNOISE3. Table 3. The effect of polymerase choice. Total number of detected sequence clusters in root nodule samples (Field-Samples-2) amplified using Phusion (proofreading) or Platinum (non-proofreading) polymerases. Sequences were clustered using MAUI-seq, DADA2, and UNOISE3. Table 3. The effect of polymerase choice. Total number of detected sequence clusters in root nodule samples (Field-Samples-2) amplified using Phusion (proofreading) or Platinum (non-proofreading) polymerases. Sequences were clustered using MAUI-seq, DADA2, and UNOISE3. Table 3. The effect of polymerase choice. Total number of detected sequence clusters in root nodule samples (Field-Samples-2) amplified using Phusion (proofreading) or Platinum (non-proofreading) polymerases. Sequences were clustered using MAUI-seq, DADA2, and UNOISE3. Table 3. The effect of polymerase choice. Total number of detected sequence clusters in root nodule samples (Field-Samples-2) amplified using Phusion (proofreading) or Platinum (non-proofreading) polymerases. Sequences were clustered using MAUI-seq, DADA2, and UNOISE3. Table 3. The effect of polymerase choice. Total number of detected sequence clusters in root nodule samples (Field-Samples-2) amplified using Phusion (proofreading) or Platinum (non-proofreading) polymerases. Sequences were clustered using MAUI-seq, DADA2, and UNOISE3. Table 3. The effect of polymerase choice. Total number of detected sequence clusters in root nodule samples (Field-Samples-2) amplified using Phusion (proofreading) or Platinum (non-proofreading) polymerases. Sequences were clustered using MAUI-seq, DADA2, and UNOISE3. Table 3. The effect of polymerase choice. Total number of detected sequence clusters in root nodule samples (Field-Samples-2) amplified using Phusion (proofreading) or Platinum (non-proofreading) polymerases. Sequences were clustered using MAUI-seq, DADA2, and UNOISE3.
Platinum Platinum Platinum Phusion Phusion Phusion
Gene
MAUI- seq
DADA2
UNOISE3
MAUI- seq
DADA2
UNOISE3
rpoB Total 16 24 26 15 15 20
Reference* 9 9 7 8 9 7
Exact BLAST* 3 3 2 3 3 2
Single nt* 3 7 8 3 2 5
Other* 1 5 9 1 1 6
recA Total 9 10 12 8 9 10
Reference 5 5 4 5 5 4
Exact BLAST 0 1 1 0 1 1
Single nt 3 3 3 3 2 3
Other 1 1 4 0 1 2
nodA Total 18 14 35 17 11 34
Reference 7 10 8 9 9 9
Exact BLAST 0 1 0 0 0 0
Single nt 6 1 4 6 1 4
Other 5 2 22 2 1 21
nodD Total 20 17 46 27 24 71
Reference 10 12 12 16 16 15
Exact BLAST 0 0 0 0 0 0
Single nt 6 3 6 5 4 6
Other 4 2 28 6 3 50