Availability of supporting data
Raw Illumina reads are available in the SRA repositories with accession
numbers [SRP221010 (Synthetic mix [44] and Field-Samples-1
[45]) and SRP238323 (Field-Samples-2 [46])]. MAUI-seq scripts
are available in the GitHub repositoryhttps://github.com/jpwyoung/MAUI.
A detailed protocol for sampling, sample preparation, and read
processing is available in Additional file 1 . Scripts used for
DADA2, UNOISE3, and figure generation are available inAdditional file 3 , 4 , and 5 , respectively.
Detailed output sequences for all three methods are available inAdditional file 2 .