Aim, design, and setting
MAUI-seq is a HTAS method designed to assess genetic diversity within or across species, using global UMI-based errors rates to detect potential PCR artefacts such as chimeras and single-base substitutions. To evaluate MAUI-seq, we compared its performance the widely-used ASV clustering methods, DADA2 and UNOISE3 on DNA mixtures of two Rltstrains to assess accuracy on a set of know sequences, and two sets of environmental samples of white clover root nodules to assess the performance on a complex set of sequences.