Table 1. Total number of detected sequences in the synthetic mix samples using MAUI-seq, DADA2 and UNOISE3. The percentage of true sequences is averaged over 23 samples for Platinum (non-proofreading) and 14 samples for Phusion (proofreading). Table 1. Total number of detected sequences in the synthetic mix samples using MAUI-seq, DADA2 and UNOISE3. The percentage of true sequences is averaged over 23 samples for Platinum (non-proofreading) and 14 samples for Phusion (proofreading). Table 1. Total number of detected sequences in the synthetic mix samples using MAUI-seq, DADA2 and UNOISE3. The percentage of true sequences is averaged over 23 samples for Platinum (non-proofreading) and 14 samples for Phusion (proofreading). Table 1. Total number of detected sequences in the synthetic mix samples using MAUI-seq, DADA2 and UNOISE3. The percentage of true sequences is averaged over 23 samples for Platinum (non-proofreading) and 14 samples for Phusion (proofreading). Table 1. Total number of detected sequences in the synthetic mix samples using MAUI-seq, DADA2 and UNOISE3. The percentage of true sequences is averaged over 23 samples for Platinum (non-proofreading) and 14 samples for Phusion (proofreading). Table 1. Total number of detected sequences in the synthetic mix samples using MAUI-seq, DADA2 and UNOISE3. The percentage of true sequences is averaged over 23 samples for Platinum (non-proofreading) and 14 samples for Phusion (proofreading). Table 1. Total number of detected sequences in the synthetic mix samples using MAUI-seq, DADA2 and UNOISE3. The percentage of true sequences is averaged over 23 samples for Platinum (non-proofreading) and 14 samples for Phusion (proofreading). Table 1. Total number of detected sequences in the synthetic mix samples using MAUI-seq, DADA2 and UNOISE3. The percentage of true sequences is averaged over 23 samples for Platinum (non-proofreading) and 14 samples for Phusion (proofreading). Table 1. Total number of detected sequences in the synthetic mix samples using MAUI-seq, DADA2 and UNOISE3. The percentage of true sequences is averaged over 23 samples for Platinum (non-proofreading) and 14 samples for Phusion (proofreading).
Platinum Platinum Platinum Phusion Phusion Phusion
MAUI- seq
DADA2
UNOISE3
MAUI- seq
DADA2
UNOISE3
exp. seq*
rpoB n seq 2 3 4 2 2 2 2
%true* 100 96.96 93.80 100 100 100 -
Cor.exp/obs* 0.956 0.977 0.981 0.996 0.999 0.9998 -
chim.freq* 0 0.07 0.13 0 0 0 -
recA n seq 2 2 2 2 2 2 2
%true 100 100 100 100 100 100 -
Cor.exp/obs 0.984 0.991 0.989 0.948 0.952 0.947 -
chim.freq 0 0 0 0 0 0 -
nodA n seq 6 5 4 5 2 4 2
%true 99.04 89.70 89.93 99.03 100 90.43 -
Cor.exp/obs 0.985 0.998 0.999 0.989 0.999 0.999 -
chim.freq 0.10 0.25 0.22 0.04 0 0.16 -
nodD n seq 7 6 21 3 3 14 3
%true 98.49 93.93 90.10 100 100 79.83 -
Cor.exp/obs 0.998 0.998 0.995 0.990 0.998 0.995 -
chim.freq 0.05 0.05 0.13 0 0 0.11 -
all %true-overall* 99.76 93.73 91.93 99.74 100 91.71 -