Th-T fluorescence assay
Th-T fluorescence assay was performed for monitoring of aggregation kinetics (Ismael et al. 2018) (Singh et al. 2017). The assay was performed in a corning 96 well flat black bottom plate (catalogue no. CLS3603). The ratio of protein samples to the Th-T solution (1 mM) was maintained at 1:1 (v/v ). The fluorescence reading was performed at an excitation-emission wavelength of 450/482 nm using a microplate reader (TECAN Infinite 200 pro) (Pandey et al. 2012). All the procedures for the Th-T fluorescence experiment were conducted in the dark condition to prevent the fluorescence decay.