Effect of shear on the aggregation of BSA
After conducting the stability study, we chose three temperature (55, 60 and 65°C) to perform the shear study at a constant shear rate of 300 s-1 (thermomechanical set-ups). For this study, a MCR 72 Rheometer with an inbuilt peltier temperature control was used. Before starting the experiment, motor adjustment of the instrument was performed to overcome erroneous data. Next, the required temperature was set and 2 ml sample of BSA solution (75µM) was placed on the lower plate of a parallel plate geometry (PP50). Then slowly the upper plate was bring down to a gap of 0.5 mm standard distance between the two plates. Samples were then sheared at 300 s-1 for a duration of 120 minutes and aliquotes of samples were collected at different time intervals to monitor the aggeragtion behavior.
For analyzing the flow behaviour of BSA solution at different temperatures, a linear shear ramp was performed from 1 to 1000 s-1 at a rate of 5 points/shift and a constant duration of 6 seconds was miantained at a shear rate.