Thermal stability study of BSA
In order to determine the stability of BSA, temperature sweep test was conducted for the three ranges 25-50°C, 25-65°C and 25-75°C at the rate of 1°C/ min using a UV visible spectrophotometer (Cary 300 bio). Also hysteresis loop scan of the above three ranges was performed to see reversibility of the protein structural changes at respective temperature. Freshly prepared 1 ml BSA solution (0.5 mg/ml) was added in a quartz cuvette and placed inside the Cary instrument. The samples were collected at three points including the initial temperature (referred as “ONE”), the maximum temperature (referred as “TWO”) and back to the initial temperature (referred as “THREE”). The samples were examined for the aggregation behavior of BSA.