Thermal stability study of BSA
In order to determine the stability of BSA, temperature sweep test was
conducted for the three ranges 25-50°C, 25-65°C and 25-75°C at the rate
of 1°C/ min using a UV visible spectrophotometer (Cary 300 bio). Also
hysteresis loop scan of the above three ranges was performed to see
reversibility of the protein structural changes at respective
temperature. Freshly prepared 1 ml BSA solution (0.5 mg/ml) was added in
a quartz cuvette and placed inside the Cary instrument. The samples were
collected at three points including the initial temperature (referred as
“ONE”), the maximum temperature (referred as “TWO”) and back to the
initial temperature (referred as “THREE”). The samples were examined
for the aggregation behavior of BSA.