Th-T fluorescence assay
Th-T fluorescence assay was performed for monitoring of aggregation
kinetics (Ismael et al. 2018)
(Singh et al. 2017). The assay was
performed in a corning 96 well flat black bottom plate (catalogue no.
CLS3603). The ratio of protein samples to the Th-T solution (1 mM) was
maintained at 1:1 (v/v ). The fluorescence reading was performed
at an excitation-emission wavelength of 450/482 nm using a microplate
reader (TECAN Infinite 200 pro) (Pandey et
al. 2012). All the procedures for the Th-T fluorescence experiment were
conducted in the dark condition to prevent the fluorescence decay.