Figure 2. (a) Far-UV CD spectra showing the secondary structural conformations of the BSA solution at 55°C (black), 65°C (red) and 75°C (blue) with reference to native (green) BSA. Spectrum of native BSA in Tris-HCl buffer (pH 7.4) was recorded at room temperature without any thermal treatment. (b) MRE signals at 222 nm for the above thermal treated samples to monitor the changes in helical content.