Determination of hydrodynamic diameter
The DLS experiment was carried out to analyze the hydrodynamic diameter
(dH ) using the litesizer 500 (Anton Paar
instrument) at room temperature. The number intensity was used for
determining the dH . The time dependent
fluctuation in the light intensity being scattered by the particles in
the solution was measured by the instrument at a fixed angle of 175°.
The dH of the particles was determined from the
Stoke-Einstein equation as \(d_{H}=KT/3\pi\eta D\). Here Kdenotes the Boltzmann constant, T is the absolute temperature in
K, η is the viscosity and D is the diffusion coefficient.
The aliquoted samples were diluted to around 1 mg/ml using filtered
Milli-Q water.