Preparation of BSA solution
Firstly, 0.5 mM stock solution of lyophilized BSA powder was prepared in 20 mM Tris-HCl buffer (pH 7.4). The solution was filtered through a 0.2 µL syringe filter and the absorbance was recorded at 280 nm using a UV spectrophotometer to estimate the final stock concentration using Beer Lambert’s law at an extinction coefficient value of 43,824 M-1 cm-1(Bekard et al. 2012). A 75 µM (5 mg/ml) working concentration was then prepared prior the experiments.