Figure 2. (a) Far-UV CD spectra showing the secondary
structural conformations of the BSA solution at 55°C (black), 65°C (red)
and 75°C (blue) with reference to native (green) BSA. Spectrum of native
BSA in Tris-HCl buffer (pH 7.4) was recorded at room temperature without
any thermal treatment. (b) MRE signals at 222 nm for the above
thermal treated samples to monitor the changes in helical content.