Animals
Sprague-Dawley rats (male, 180 ~ 220g, License No. SCXK
2016-0002) and Kunming mice (male, 18 ~ 22 g, License
No. SCXK 2016-0002) were all purchased from Hunan SJA Laboratory Animal
Co., Ltd (China). Animals were kept under controlled conditions (12 h
dark/12 h light cycle, water and food supply ad libitum). For the
characterization of thrombosis inhibition of compounds in rats deep
venous thrombosis model, either compound or vehicle (normal saline, NS)
was administered subcutaneously after randomization (n=6 respectively).
For studying bleeding risk of compounds in mice tail-bleeding model,
either compound or NS was administered subcutaneously after
randomization (n=8 respectively). For characterizing pharmacodynamics of
dHG-5 in mice, dHG-5 was administered subcutaneously after randomization
(n=6 respectively).