Drugs and reagents
All salts for Krebs solutions were of AnalaR grade or higher and
obtained from BDH, Poole or Fisons, Loughborough. Chloroquine
diphosphate, (±) adrenaline HCl, ethyl carbamate (urethane), Tween 80,
N,N-dimethylacetamide and propylene glycol were obtained from Sigma,
Poole. Heparin sodium (Multiparin 5000 IU ml-1)
injection was obtained from CP Pharmaceuticals, Wrexham. MTBE was
purchased from Fisons, Loughborough; Ro5-4864 was purchased from Fluka
Chemika, Gillingham, Dorset; Hypnorm (fentanyl / fluanisone) was from
Janssen Animal Health, Petteridge, Kent; Amprol Plus (Amprolium HCl /
ethopabate) was from Merk Sharp & Dohme, Hertfordshire; sodium
pentobarbitone from RMB Animal Health Ltd, Dagenham.
7-chloro-4-(5-diethylamino-1-methylpentyl-amino)-quinoline diphosphate
was a gift from the Walter Reed Army Institute of Research, Washington
D.C.; and clonazepam and diazepam were gifts from Hoffman-La Roche,
Basel.