HPV genotyping
HPV genotyping was done using SACACE HPV High Risk and Low Risk Typing Real-TM kit (SACACE, Italy). This method enables the detection on twelve High Risk HPV (HR-HPV) genotypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58 and 59) and two Low Risk HPV (LR-HPV) (6, 11) targeting E7 region.
The strips then were inserted into the Strata-gene MX3000P for the amplification step. The amplification programmed was shown in Table 2.
Results obtained from RT-PCR were then keyed-in to the software (Microsoft® Excel HPV Typing Real-Time MX Results Matrix.xls) provided by the SACACE kit.