Figure 5: The ARR4 c.569C>G (p.S190*) mutation destabilized ARR4 via the proteasome system. (A) APRE19 cells transfected with wild-type and mutated ARR4 with flag tags were treated with 0 μM, 25 μM, and 50 μM of MG132 for 24h and the ARR4protein levels detected. The APRE19 cells were simultaneously treated with CQ(chloroquine). Actin protein levels served as the as endogenous control.