Figure 2: The ARR4 mutation identified in myopia patients. (A) Sanger sequencing analysis for the mutation identified in the family with high myopia. (B) Location of the pathogenic ARR4protein. The protein contains two important functional domains: an arrestin_N domain and an arrestin_C domain. (C) The nonsenseARR4 c.569C>G (p.S190*) mutation is highly conserved among different species. (D) The pedigree for the family with the c.569C>G (p.S190*) mutation. Square = male, circle = female, dark symbol = affected, arrow = proband, slash = deceased member, ‘Ω’ = samples used for exome sequencing, ‘+’ = DNA available for this study.