The mutant ARR4 protein mediated ER-stress.
To verify whether the accumulation of mutant protein mediated ER-stress
occurred, the expression of the relevant protein markers were examined.
Notably, in the cells transfected with c.569C>G (p.S190*)
mutant plasmids, the expression levels of ER stress markers such as
CHOP, XBP1, ATF6, and GRP78 were significantly increased (Figure 4).
This suggested that mutant ARR4 is associated with mediating ER-stress.