Ethical Approval and Study design
All proceedings performed in this study have been approved by local ethical committee from Universidade Brasil (registration number 3.020.830).
Fifty volunteers (25 sedentary civil individuals working into a normal diurnal fixed working day; 8 hours per day; ≥5 years; 34,00±9,60 years old) and (25 sedentary military policemen working in a shiftwork scale 12 x 24 hours and 12 x 48 hours; ≥5 years; 38,73±6,92 years old) were recruited and took part in the study, after agreement and signature of the term of consent. To be included into the study, the policemen must have at least ≥5 years working as policemen in the presented working scale, while the civil men should not have worked at least for 5 years into a working scale that not in a fixed time (diurnal and for maximal 8 hours per day and between 36 to 44 hours per week).
All volunteers were classified as non-obese, civil (BMI = 26,30±3,85) and policemen (BMI = 28,58±5,28), non-hypertensive, non-diabetics, without dyslipidemia, no cardiovascular diseases, never smokers and without any previous or current respiratory diseases. In addition, a complete body composition analysis using multifrequency octopolar bioimpedance equipment, Bioscan 920 II S (Maltron Int, UK) was performed.