Western blot
Cells were lysed in radio immunoprecipitation assay (RIPA) lysis buffer
as described previously (Li et al., 2014). The whole cell lysates were
collected and separated by 10% SDS-PAGE and subsequently
electro-transferred onto a polyvinylidene difluoride membrane (Millipore
Corp., Bedford, MA). The blocked membrane was incubated with the
indicated antibodies. Final detection was performed using a
chemiluminescent substrate system (Cell signaling, CA).