Reagents
SBF-1 is a steroidal glycoside, which is synthesized by the co-author
Prof. Biao Yu, and its structure was shown in Fig. 1A. Primary
antibodies (AR, rabbit monoclonal, Cat# 5153T, RRID:AB_10691711),
(phosphorylated AKTS473, rabbit polyclonal, Cat# 9271,
RRID:AB_329825), (AKT1, rabbit monoclonal, Cat# 2938,
RRID:AB_915788), (Phosphorylated FOXO1S256, rabbit
polyclonal, Cat# 9461, RRID:AB_329831), (FOXO1, mouse monoclonal,
Cat# 97635, RRID:AB_2800285), (Bcl-2, mouse monoclonal, Cat# 15071,
RRID:AB_2744528), (PCNA, rabbit monoclonal, Cat# 13110,
RRID:AB_2636979) were purchased from Cell Signaling Technology
(Beverly, MA). Primary antibody (Phosphorylated ARS515,rabbit polyclonal, Cat# ab128250, RRID:AB_11141430) were purchased
from Abcam (China). Primary antibody (IGF-1, rabbit polyclonal, Cat#
PA1-86913, RRID:AB_2122127) were purchased from Thermo scientific.
Primary antibodies (β-Actin, mouse monoclonal, Cat# sc-47778 HRP,
RRID:AB_2714189), (GAPDH, mouse monoclonal, Cat# sc-32233,
RRID:AB_627679) was purchased from Santa Cruz Biotechnology (Santa
Cruz, CA).
3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) and
dimethyl sulfoxide (DMSO) were from Sunshine Biotechnology (Nanjing,
China). 5α-Dihydrotestosterone (DHT) solution were purchased from
Sigma-Aldrich (St. Louis, MO). All the plasmids: pcDNA3.1
[Flag-ARWT, Flag-AR∆DBD, GFP-
ARWT, GFP- ARL702H, GFP-
ARW742C, GFP- ARF876L, and GFP-
ART878A] were purchased from Gene-Script (Nanjing,
China). All other chemicals were purchased from Sigma-Aldrich (St.
Louis, MO).