2.1 Yeast cultivation
The strain S. cerevisiae YL-T3 (Wang et al., 2018) was grown in
YPD medium (containing 10 g/L of yeast extract, 20 g/L of beef peptone,
20 g/L glucose). The recombinant yeast transformants were selected on
SC-His-Leu-Ura (containing 20 g/L of glucose, 6.7 g/L of YNB, 0.65 g/L
of Do-His-Leu-Ura3) plates at 30 °C (Dai et al., 2012), and these
transformants were grown in SC-His-Leu-Ura liquid medium at 30 °C and
220 rpm