Figure Legends
Figure 1. Influence of BMI on fasting C3, C3adesArg and TCC concentrations in PCOS and control subjects (cohort 1).
Boxplots of C3 (A), C3adesArg (B) and TCC levels in PCOS subjects and metabolically healthy controls. Data grouped according to BMI (lean: BMI 18.5-24.99; overweight: BMI 25-29.99 and obese: BMI≥30 kg/m2). Middle lines show the medians of the data and the boxes highlight the 25th and 75th percentiles (Quarter 1 and Quarter 3). C3 levels were significantly higher in obese subjects with PCOS compared to obese controls (mean difference ± SEM; 0.222 ± 0.099 g/l, p < 0.05). TCC: terminal complement complex.
Figure 2. Effects of postprandial lipaemia on triglyceride and complement concentrations in PCOS and control subjects.
Postprandial triglyceride (A), factor H (B), TCC (C), C3 (D), C4 (E), properdin (F) and C3adesArg (G) levels in response to OFTT in PCOS cohort and control groups (cohort 2). Data are mean ± SEM. Significant differences between baseline and postprandial circulating levels in PCOS or control groups are represented by asterisk or open circle, respectively. Significant differences between PCOS and control groups are denoted by the hash symbol. In all cases *, o,# represent p< 0.05; **, o o, # # represent p< 0.001 and ***,o o o, # # # represent p< 0.0001.
Figure 3. Influence of obesity on factor H, C3 and TCC responses to postprandial lipaemia.
Postprandial factor H (A), TCC (B) and C3 (C) responses to OFTT in obese (BMI ≥ 30 kg/m2) and non-obese (BMI < 30 kg/m2) PCOS and control subjects (cohort 2). Significant differences between obese and non-obese PCOS subjects are denoted by an asterisk (* p< 0.05; ** p< 0.001; *** p< 0.0001). No significant differences were observed between obese and non-obese controls.
Figure S1. Relationship between factor H area under the curve (AUC), body fat and metabolic parameters.
Figure S2. Correlation between fasting factor H concentrations and BMI. Data represent correlations between factor H levels and BMI in different age groups in PCOS subjects (triangles, dashed line) and controls (circles, solid line) (cohort 2). Factor H levels correlated positively with BMI in both PCOS and controls in all age groups.
Table 1. Fasting plasma complement levels in cohort 1.