2.2 Mitochondrial genome sequencing
For mitochondrial genome sequencing, the Miseq platform was used and
more than 1 Gb were sequenced in each sample. To assemble these data,
CLC Assembly Cell package (version 4.2.1) was used. After trimming raw
data using CLC quality trim (ver. 4.21), assembly was accomplished using
the CLC de novo assembler with dnaLCW. Assembled sequences were
confirmed by BLASTZ(Schwartz et al., 2003). The GeSeq program was used
for annotation (Tillich et al., 2017) and the result manually checked
based on alignment of other Spodoptera species mitochondrial
genomes using MEGA 7 (Kumar, Stecher, & Tamura, 2016). The circular
mitochondrial genome map was generated by OGDraw program (Lohse,
Drechsel, & Bock, 2007).