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Spike development inhibition in the ftin mutant is associated with multiple phenotypic characteristics and regulated multiple biological pathways
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  • Yong-sheng Zheng,
  • Jinpeng Zhang,
  • Cheng Liu,
  • Rongzhi Zhang,
  • Xiajie Ji,
  • Han Zhang,
  • Ruyu Li,
  • Weihua Liu
Yong-sheng Zheng
Chinese Academy of Agricultural Sciences
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Jinpeng Zhang
Chinese Academy of Agricultural Sciences
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Cheng Liu
Shandong Academy of Agricultural Sciences
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Rongzhi Zhang
Shandong Academy of Agricultural Sciences
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Xiajie Ji
Chinese Academy of Agricultural Sciences
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Han Zhang
Shandong Academy of Agricultural Sciences
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Ruyu Li
Shandong Academy of Agricultural Sciences
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Weihua Liu
Chinese Academy of Agricultural Sciences
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Peer review status:UNDER REVIEW

13 Apr 2020Submitted to Plant, Cell & Environment
21 Apr 2020Assigned to Editor
21 Apr 2020Submission Checks Completed
23 Apr 2020Review(s) Completed, Editorial Evaluation Pending

Abstract

Spike development of wheat is closely associated with the ability of response to cold stress and inhibited under cold stress in spring. Morphological investigation showed that the ftin gene in 3558M is associated with multiple phenotypic characteristics, including fewer tillers, delayed floral transition, and the death of shoot apical meristem. In this study, we systematically researched the genetic nature of spike development using ITRAQ, transcriptome sequencing, western blot and RNAi technologies. The results showed that the ftin mutant is cold sensitive and activates the cold acclimation pathway. Multiple defence responses, including the ROS-mediated hypersensitive response, SA-mediated systemic acquired resistance and FLS2-induced pathogen-associated defence response, were activated to respond to normal cold stress and led to the apex death. Meanwhile, the continuous cold acclimation pathway inhibited the SVP-SCO1-LFY flowering pathway and leads to inhibition of spike development. Two TaPIN proteins were significantly downregulated, and multiple auxin signalling genes were also differentially expressed after cold stress. We used RNAi technology to knock down the two TaPIN genes and the tiller number was significantly reduced in TaPINa-RNAi and TaPINb-RNAi wheat. Taken together, the results revealed that the ftin gene might directly or indirectly regulate multiple biological pathways to affect multiple phenotypic characteristics.