Bone resorption assay
Functional evidence of osteoclast formation was determined via a lacunar
resorption assay system using cell cultures on dentine slices as
previously described(17). Cells were removed from the dentine slices by
treatment with 0.1 M ammonium hydroxide. The dentine slices were washed
in distilled water and ultrasonicated to remove adherent cells, then
stained with 0.5% (v/v) toluidine blue to reveal areas of lacunar
resorption, and examined via light microscopy.