Quantitative PCR
Total RNA was extracted using TRIzol reagent (Invitrogen), and 1 μg of
total RNA was reverse-transcribed into cDNA using a FastQuant RT Kit
(with gDNase) (Tiangen Biotech). RT-PCR was carried out using the
QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) with
Talent qPCR PreMix (SYBR Green) (Tiangen Biotech). Data were normalized
to the expression of GAPDH. The primer nucleotide sequences for PCR were
obtained from GenBank database and synthesized by Sangon Biotech
(China). The primer sequence information used in the study was shown in
the supplementary material, Table1.