Phylogeography
The inferred networks show a close genetic similarity between Floreana and Champion populations of both lizard species. In contrast, samples from Gardner are markedly different (Fig. 3). For lava lizards, the full dataset resulted in a single haplotype each for Floreana (including the single specimen from Black Beach), Gardner and Champion; where Champion and Gardner are separated from Floreana by one and 23 mutations, respectively. After excluding identical sequences (five from Gardner— QCAZ 16769, 16770, 16773, 16776, 16781—and three from Champion— QCAZ 16760, 16762, 16764), two haplotypes each for Floreana (Black Beach and Post Office Bay) and Champion are recovered; the single Gardner haplotype is separated from the others by at least 41 mutations. For leaf-toed geckos, the full dataset resulted in four haplotypes for Floreana, one for Champion, and three for Gardner, which are separated from other haplotypes by a minimum of 11 mutations. Haplotypes from Floreana are separated from Champion’s haplotypes by at least three mutations. After excluding identical sequences (two from Champion— QCAZ 16704, 16707—, one from Gardner— QCAZ 16719), the leaf-toed gecko network is only different in that Gardner’s haplotypes are separated from other haplotypes by at least 12 mutations.