Phylogeography
The inferred networks show a close genetic similarity between Floreana
and Champion populations of both lizard species. In contrast, samples
from Gardner are markedly different (Fig. 3). For lava lizards, the full
dataset resulted in a single haplotype each for Floreana (including the
single specimen from Black Beach), Gardner and Champion; where Champion
and Gardner are separated from Floreana by one and 23 mutations,
respectively. After excluding identical sequences (five from Gardner—
QCAZ 16769, 16770, 16773, 16776, 16781—and three from Champion— QCAZ
16760, 16762, 16764), two haplotypes each for Floreana (Black Beach and
Post Office Bay) and Champion are recovered; the single Gardner
haplotype is separated from the others by at least 41 mutations. For
leaf-toed geckos, the full dataset resulted in four haplotypes for
Floreana, one for Champion, and three for Gardner, which are separated
from other haplotypes by a minimum of 11 mutations. Haplotypes from
Floreana are separated from Champion’s haplotypes by at least three
mutations. After excluding identical sequences (two from Champion—
QCAZ 16704, 16707—, one from Gardner— QCAZ 16719), the leaf-toed
gecko network is only different in that Gardner’s haplotypes are
separated from other haplotypes by at least 12 mutations.