2-NBDG uptake assay
The cells were seeded into a 24-well glass bottom plate, and incubated
at 37 oC in a humidified incubator with 5%
CO2 until reached a density of 60%-70%. Changed with a
serum-free medium for two hours before taking experiment. After that,
added 150 μL sodium-HEPES buffer containing 240 μM 2-NBDG and 150 μL
compound solutions to the plate to a final concentration of 10 μM.
Incubated cells at 37 oC for 30 min. Then, washed
cells with sodium-HEPES buffer three times and photographed with a Zeiss
LSM laser confocal imaging system.