RNA extraction and RT-PCR
Primer sequences were synthesized by Generay Biotech and the total RNA
was extracted from cells using RNAiso Plus kit (Takara, Japan). The mRNA
levels of SLC5A1 , SLC5A2 , and Actin were detected
by the quantitative real time PCR. The primer sequences of SGLT1 and
SGLT2 are as follows: SLC5A1-F: GTCGGACTGTGGGCTATGTT, SLC5A1-R:
GTCTGCAAGGTGTCCGTGTA; SLC5A2-F: ACGCCCATGTTTCTCATGGT, SLC5A2-R:
CCTGGGGCTCATTCATCTCC.