Statistics analysis
The data and statistical analyses were complied with the recommendations on experimental design and analysis in pharmacology (Curtis et al., 2018). Data were expressed as the mean ± SEM. Data between two groups were analyzed by t-test using Graphpad Prism (Graphpad Software Inc, California, USA). Statistical analyses for multiple groups was performed by one-way ANOVA followed by Tukey’s HSD post hoc tests. A pvalue of ≤ 0.05 was considered statistically significant.