The anti-diabetic effect of CAG is related to the inhibition of
SGLT2
Considering that the antidiabetic effect and manner of CAG were
similar to that of Dapagliflozin, we further studied the effect ofCAG on SGLT protein. By using an immunohistochemistry
detection, a higher expression of SGLT2 was observed in kidney of ZDF
diabetic rats in the vehicle group compared with the lean mice, and a
reversal effect of Dapagliflozin was also overserved, which was
consistent with the previous reports. A similar decreasing was found in
the CAG -treated diabetic rats (Figure 7A). Similarly,CAG and Dapagliflozin also inhibited the expression of SGLT2 in
the HEK293 cell lines with over-expression of SGLT2 (Figure 7B and 7C).