2.3. Multiple sequence alignment and phylogenetic analysis
The genome sequences of PCV4 obtained in this study have been deposited
in GenBank under the accession numbers MT311852–MT311854. Multiple
sequence alignments were carried out using the Megalign program within
the Lasergene package (DNAStar software, DNASTAR Inc.), and phylogenetic
relationships were assessed with MEGA software (version 7). Support for
the phylogenetic relationships was determined by bootstrapping (1000
replicates). In the present study, the method described by Zhang et al.
was used to divide the clades of PCV4 (Zhang et al., 2019).