2.3. Multiple sequence alignment and phylogenetic analysis
The genome sequences of PCV4 obtained in this study have been deposited in GenBank under the accession numbers MT311852–MT311854. Multiple sequence alignments were carried out using the Megalign program within the Lasergene package (DNAStar software, DNASTAR Inc.), and phylogenetic relationships were assessed with MEGA software (version 7). Support for the phylogenetic relationships was determined by bootstrapping (1000 replicates). In the present study, the method described by Zhang et al. was used to divide the clades of PCV4 (Zhang et al., 2019).