Figure 3 Phylogenetic analysis based on the nucleotide sequences corresponding to the complete genome
Note: GETV isolated in the present study is indicated by ●; Bootstrap values (based on 1000 replicates) for each node are given if >60%.
Analysis using MegAlign software showed that GETV-V1 had 36 unique nucleotide mutations in its genome, including 35 nucleotide substitutions and 1 nucleotide insertion (Table 2). Interestingly, only 15 nucleotide mutations resulted in amino acid substitutions. These mutations with the nucleotide insertion in the 3′ UTR of the genome could be a unique hallmark of GETV-V1.
Table 2 Comparison of genomic sequences of GETV-V1 strain with reference strains