loading page

Isolation and characterization of the first porcine Getah virus strain HNJZ-S1 from an aborted piglet in China
  • +4
  • Feng Zhou,
  • Aojie Wang,
  • Hongtao Chang,
  • Dandan Cui,
  • Xingang Wang,
  • Lu Chen,
  • chuan-qing WANG
Feng Zhou
Henan Agricultural University
Author Profile
Aojie Wang
Henan Agricultural University
Author Profile
Hongtao Chang
Henan Agricultural University
Author Profile
Dandan Cui
Henan Agricultural University
Author Profile
Xingang Wang
Henan Agricultural University
Author Profile
Lu Chen
Henan Agricultural University
Author Profile
chuan-qing WANG
Henan Aricultural University
Author Profile

Abstract

Severity of porcine reproductive and respiratory syndrome (PRRS) implicated co-infection with other pathogens in pig herd in China, which normally were found Japanese encephalitis virus (JEV), pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus, porcine parvovirus and classical swine fever virus (CSFV). Here we verified a specific fragment which was similar to Getah virus (GETV) by RT-PCR and sequencing from aborted fetuses in Henan province, China. Then we isolated and purified the stain virus. We named it as HNJZ-S1. Furthermore, we characterized HNJZ-S1 by passage and plaque titer, whole genome sequencing, Electron microscopy and animal infection experiments with mice and piglets. The data show that the full-length genome sequence of HNJZ-S1was 11689 bp with homologous 97.4%–99.3% identical to the GETV sequence available in GenBank. Phylogenetic tree analysis showed that HNJZ-S1 was closely related to mosquito isolate 12IH26 from Japan, horse isolates 14-I-605-C1 and 14- I-605-C2, and mosquito isolate HB0234 from China. Animal experiments showed that the isolate HNJZ-S1 caused abortion in pregnant mice and was lethal postnatal day 3 mice and piglets. This study provides important information on the tropism, infection, pathogenicity, environmental microbiology and public health implications of pig derived GETV and will also serve as a source of reliable research materials.