The whole viral genomic sequencing and phylogenetic analysis
The viral genome was amplified in fragments using specific primers reported previously [16], and the non-coding region was amplified according to the instructions of the 5’Full RACE and 3’Full RACE Core Set Ver.2.0 kits (Takara Biomedical Technology Co., Ltd, Dalian). After each fragment was ligated into the pMD18-T vector, Escherichia coli DH5α cells were transformed with recombinant plasmids. All the fragments were sequenced by Sangon Biotech Co., Ltd (Shanghai).
The sequenced fragments were spliced using DNAMAN software. Sequence similarity and phylogenetic analyses were conducted using DNAStar and MEGA4.0. Furthermore, the amino acid sequences of GETV E2 genes were compared and homology was analyzed using MegAlign. Phylogenetic analysis of amino acid sequences derived from the E2 gene were conducted using neighbor-joining methods (1000 bootstrap replicates) in MEGA4.0.