Verification and Replication of GETV in vitro
The filtered sample supernatant was used to inoculate Marc-145 cells,
and obvious CPE was observed after three generations (figure
2A ). A single plaque clone was obtained for plaque purification.
Compared with the control group, cells infected with the isolate became
rounded and partly exfoliated about 24-hour post-infection. A large
number of cells exfoliated after 48 hours, at which time most of the
adherent cells became rounded. Both RT-PCR detection and sequencing
results confirmed the isolate as GETV, and it was named HNJZ-S1. Plaque
forming unit show round (Figure 2B ). Monitoring of
proliferation showed that the virus titer peaked at
107.25 TCID50/mL around 36 h
post-inoculation Marc-145 cells, and then began to decrease slowly
(figure C ).