Verification and Replication of GETV in vitro
The filtered sample supernatant was used to inoculate Marc-145 cells, and obvious CPE was observed after three generations (figure 2A ). A single plaque clone was obtained for plaque purification. Compared with the control group, cells infected with the isolate became rounded and partly exfoliated about 24-hour post-infection. A large number of cells exfoliated after 48 hours, at which time most of the adherent cells became rounded. Both RT-PCR detection and sequencing results confirmed the isolate as GETV, and it was named HNJZ-S1. Plaque forming unit show round (Figure 2B ). Monitoring of proliferation showed that the virus titer peaked at 107.25 TCID50/mL around 36 h post-inoculation Marc-145 cells, and then began to decrease slowly (figure C ).