Animal infections
Ten gestation Kunming mice at day 10 with GETV negative by oral and anal
swab tests, 120 mice at the age of postnatal day 3, and Ten 3-day-old
piglets without colostrum were randomly divided into two groups,
respectively. One group were lightly anesthetized with isoflurane and
intranasally inoculated with 106TCID50 of virus in 100ul DMEM, while pure DMEM was used
for the other group as control. Animal were monitored for signs of
infection (body weight loss, hunched posture, ruffled coat, lethargy and
dehydration, abortion and death) daily. Dead or dying animal were
examined, and Lung and spleen of newborn mice and piglets were dissected
for viral assay by RT-PCR.