GETV HNJZ-S1 has obvious pathogenicity in pregnant mice and neonatal mice the
Five pregnant mice gave the birth of 57 mummified fetuses (Figure 5A ) and miscarriages (Figure 5B ) between 3 and 6 days after exposure with HNJZ-S1, as well as 11 normal spring. Meanwhile, 67 normal offspring were born in five litters from the control group (Figure 5C ). There were no mummified fetuses or miscarriages found in the control group. Thus, composition of average litter size was significantly different between the HNJZ-S1 infection and non-infect groups (Figure 5D ). GETV was confirmed from aborted fetuses and premature offspring by RT-PCR.
Mice at age of postnatal day 3 were incubated with HNJZ-S1. All the infected group showed severe hindlimb paralysis and slow growth after three days exposure, and there were no survivals beyond 10 days after exposure (Figure 6A ). There was no obvious abnormality in newborn mice of the control group (Figure 6B ).