Animal infections
Ten gestation Kunming mice at day 10 with GETV negative by oral and anal swab tests, 120 mice at the age of postnatal day 3, and Ten 3-day-old piglets without colostrum were randomly divided into two groups, respectively. One group were lightly anesthetized with isoflurane and intranasally inoculated with 106TCID50 of virus in 100ul DMEM, while pure DMEM was used for the other group as control. Animal were monitored for signs of infection (body weight loss, hunched posture, ruffled coat, lethargy and dehydration, abortion and death) daily. Dead or dying animal were examined, and Lung and spleen of newborn mice and piglets were dissected for viral assay by RT-PCR.