Phylogenetic analysis of HNJZ-S1
Whole genome sequence alignment showed that the sequence similarity between HNJZ-S1 and most reference strains available in GenBank was 97.4–99.6%, which the lowest (97.4%) one was Sagiyama strain isolated from Japan in 1956, while the highest one was JL17-08 strain isolated from China (99.6%) (Table 2 ). Phylogenetic tree analysis of nucleotide sequence confirmed above results which HNJZ-S1 was clustered into mosquito isolates such as JL17-08, JL1707 and HB0234 isolated from China (Figure 4A ). Phylogenetic analysis of amino acid sequences derived from the E2 gene showed that HNJZ-S1 was also closely related to reference strains published in the past 30 years (Figure 4B ).