Virial particle investigation by electron microscope
Marc-145 cells were infected with HNJZ-S1 for 36 h. The cells were
collected, subjected to three freeze-thaw cycles, and centrifuged at
10,000 g for 5 min. One drop of the supernatant was added to a copper
mesh covered with Formvar film. Excess liquid was removed from the edge
of the mesh after 3 min, then the mesh was air-dried at room
temperature. The mesh was stained with negative dyeing including 2%
phosphotungstic acid hydrate (pH 7.2) for 5 min, excess staining
solution was removed with filter paper and air-dried at room
temperature. The mesh was observed under JEM-1400 transmission electron
microscopy (voltage 100 KV) (JEOL Ltd Japan).