FIGURES
Figure 1 . Time profile of viable cell density (▲), cell viability (◊), glucose (●) and lactate (■) concentration of batch cultures run in shake flasks (A1: parental KB26.5 and B1: engineered KB26.5-BHRF1). Napierian logarithm of viable cell density versus time (●) (A2: parental KB26.5 and B2: engineered KB26.5-BHRF1). Data obtained from duplicates cultures in shake flasks and the error bar correspond the standard deviation.
Figure 2 . 2-Litres bioreactor batch cultures of the Parental KB26.5 cell line (A.1 and A.2) and the engineered KB26.5-BHRF1 (B1 and B2). Time profile of cell density (▲), cell viability (◊); glucose (●), lactate (■) and product (bars) concentration of batch cultures. Duplicate cultures are shown for each cell line.
Figure 3 . 2-Litres bioreactor batch cultures of the Parental KB26.5 cell line (A.1 and A.2) and the engineered KB26.5-BHRF1 (B1 and B2). Napierian logarithm of viable cell density versus time (●). Duplicate cultures are presented for each cell line.
Figure 4 . Metabolic fluxes distribution of parental KB26.5 hybridoma cell line. The arrows indicate the direction of the reaction and the arrow’s thickness is proportional to the flux rate. Different coloring has been used for each pathway and model was structured in cytoplasmic and mitochondrial reactions (mitochondrion is limited by a discontinued line). The value therein the boxes corresponds to the flux rate in (nmol metabolite / (mg DCW·h)).
Figure 5 . Metabolic fluxes distribution of the engineered hybridoma cell line KB26.5-BHRF1. The arrows indicate the direction of the reaction and the arrow’s thickness is proportional to the flux rate. Different coloring has been used for each pathway and model was structured in cytoplasmic and mitochondrial reactions (mitochondrion is limited by a discontinued line). The value therein the boxes corresponds to the flux rate in (nmol metabolite/ mg DCW·h).
Figure 6 . Distribution of ATP generation (concerning the reaction in which ATP is consumed and generated in both cell lines KB26.5 and KB26.5-BHRF1. ATP consumption (A) and production (B) (nmol ATP/ (mg DCW·h)).