3.2.- Reduced genome-scale metabolic model
In this study, the reduced genome scale metabolic model published by
Quek et al. (2010) was further modified to adapt it to the experimental
conditions. The original model is free available in Systems Biology
Markup Language (SBML) (Martínez et al., 2013). The model was derived
from the complete Hybridoma genome-scale metabolic model constructed
from a generic genome-scale metabolic model of Mus musculus (Quek
and Nielsen, 2008; Sheikh et al., 2005). The adaptation process to
obtain a reduced model able to perform flux analysis for the experiments
performed in hybridoma is detailed in (Martínez-Monge et al., 2019a).
The base metabolic model does not contain the biomass formation
equation, since the objective function defined was the maximizing or
minimizing of the ATP yield. So, the base model was modified by adding
14 reactions involved in the synthesis of all metabolites precursors
needed for biomass formation, as detailed in Martínez-Monge et al.
(2019a). The final model is composed by 362 internal and 36 external
reactions that overall involve 395 metabolites. A complete list of all
included reactions and metabolites used is freely available in theSupplementary Data 1 in xlsx format.