2.2.- Culture medium
The basal medium used was Dulbecco’s modified Eagle’s media (DMEN)
(D5796, Sigma) containing 25 mM glucose and 4 mM glutamine. Basal media
was further supplemented with 2 mM glutamine (Sigma), 5 % FBS (v/v)
(Sigma), 0.5 % Antifoam C Emulsion (Sigma) and 2 % Kolliphor® P 188
(Sigma).