3.2.- Reduced genome-scale metabolic model
In this study, the reduced genome scale metabolic model published by Quek et al. (2010) was further modified to adapt it to the experimental conditions. The original model is free available in Systems Biology Markup Language (SBML) (Martínez et al., 2013). The model was derived from the complete Hybridoma genome-scale metabolic model constructed from a generic genome-scale metabolic model of Mus musculus (Quek and Nielsen, 2008; Sheikh et al., 2005). The adaptation process to obtain a reduced model able to perform flux analysis for the experiments performed in hybridoma is detailed in (Martínez-Monge et al., 2019a). The base metabolic model does not contain the biomass formation equation, since the objective function defined was the maximizing or minimizing of the ATP yield. So, the base model was modified by adding 14 reactions involved in the synthesis of all metabolites precursors needed for biomass formation, as detailed in Martínez-Monge et al. (2019a). The final model is composed by 362 internal and 36 external reactions that overall involve 395 metabolites. A complete list of all included reactions and metabolites used is freely available in theSupplementary Data 1 in xlsx format.