2.4. Characterization of lipase immobilization on PHB supports
Different concentrations of LipM7-PhaP dissolved in 10 mM sodium phosphate (pH 7.4) were immobilized on PHB nanofibers and PHB granules (average diameter: 2.5 μm). The amount of LipM7-PhaP immobilized on the PHB support was estimated by quantifying unbound proteins. The maximum loading capacity (q max) and the dissociation constant (k d) were determined by applying the protein concentration to the Langmuir isotherm model.