3.4. Effect on the expression of VEGFR2 and CD34:
Immunohistochemical analysis showed that that EAC control group had high
expression of VEGFR2, the main responder to VEGF signal that regulates
endothelial migration and proliferation. Significant reduction in
expression was observed in cisplatin treated group (p<0.001).
Treatment with the test compounds in both doses decreased the expression
compared to control EAC (p<0.001), but was still significantly
higher than the cisplatin treated group (Fig. 4) . The highest
expression of CD34 was observed in the control EAC group. All other
treated groups showed significantly lowered expression compared to
control (p<0.001). Expression of CD34 in the group treated
with the higher dose of benzimidazotriazine (10mg/kg) was not
significantly different from that of the cisplatin treated group