2.7.1. Study design:
Animals were randomly divided into 7 groups, 10 animals each. Group (1)
received the vehicle PBS (phosphate buffer saline) and was considered as
normal group. Group (2) served as the EAC control untreated group. Group
(3) received the Cisplatin as a standard drug (i.p.) at a dose of 2mg/kg
(Williams et al., 2001 ). Groups (4 & 5) received
benzoimidazoquinazoline (i.p.) at doses of 5 mg/kg and 10 mg/kg
respectively. Groups (6 & 7) received benzimidazotriazin (i.p.) at
doses of 5 mg/kg and 10 mg/kg respectively. The synthetic compounds were
dissolved in PBS as a vehicle. Cisplatin was available as vials ready to
use. All treatments started at the 7th day after
inoculation EAC cells and were continued daily for 14 days except for
cisplatin which was given every 3 days for two weeks.