3.4. Effect on the expression of VEGFR2 and CD34:
Immunohistochemical analysis showed that that EAC control group had high expression of VEGFR2, the main responder to VEGF signal that regulates endothelial migration and proliferation. Significant reduction in expression was observed in cisplatin treated group (p<0.001). Treatment with the test compounds in both doses decreased the expression compared to control EAC (p<0.001), but was still significantly higher than the cisplatin treated group (Fig. 4) . The highest expression of CD34 was observed in the control EAC group. All other treated groups showed significantly lowered expression compared to control (p<0.001). Expression of CD34 in the group treated with the higher dose of benzimidazotriazine (10mg/kg) was not significantly different from that of the cisplatin treated group