Assays of rasagiline
Blood samples were centrifuged at 2500g a t 2-8°C for 10 minutes to obtain plasma samples. The plasma samples were stored at -80°C which met the conditions for storage of stable samples in the validation of methodology. Plasma concentrations of rasagiline were analyzed using a validated liquid chromatography-mass spectrometry method (LC-MS/MS). The linear range was 20.0 to 20000 pg/mL for rasagiline. The lower limit of quantification was 20.0 pg / ml. The precision range of each concentration quality control sample (% CV) was 3.5%-6.5%. The mean bias (%) from the theoretical concentration of QC samples was between-0.5% and 3.3% for rasagiline.