Assays of rasagiline
Blood samples were centrifuged at 2500g a t 2-8°C for 10 minutes to
obtain plasma samples. The plasma samples were stored at -80°C which met
the conditions for storage of stable samples in the validation of
methodology. Plasma concentrations of rasagiline were analyzed using a
validated liquid chromatography-mass spectrometry method (LC-MS/MS). The
linear range was 20.0 to 20000 pg/mL for rasagiline. The lower limit of
quantification was 20.0 pg / ml. The precision range of each
concentration quality control sample (% CV) was 3.5%-6.5%. The mean
bias (%) from the theoretical concentration of QC samples was
between-0.5% and 3.3% for rasagiline.