Visualization of Na+ ion specific fluorescence
in leaves and roots of P. pinnata
To investigate the pattern of Na+ ion accumulation in
leaves and roots of P. pinnata , a cell permeable
Na+ specific fluorescent probe CoroNa-Green AM was
used (Supplementary Figure 3A-J and Supplementary Figure 4A-T ). Leaf
sections of control plants were showed no strong Na+specific fluorescence signal at 1, 4 and 8DAS. Similarly, leaf sections
of 300 mM NaCl treated plants were also showed no strong
Na+ specific fluorescence signal at 1 and 4DAS,
whereas a Na+ specific green florescence was observed
in apoplasmic region at 8DAS. Conversely, we could observe a consistent
increase in the Na+ fluorescence signal in apoplasmic
regions in leaf sections of 500 mM NaCl treated plants at 1, 4 and 8DAS.
Further, roots were followed a different pattern of fluorescence in both
control and salt treated plants. We could observe no strong fluorescence
signal in the cortical apoplasmic regions of control root sections at 1,
4 and 8DAS. However, we could observe a strong fluorescence signal in
the cortical apoplasmic regions dose dependently in both 300 and 500 mM
NaCl treated root sections at 1, 4 and 8DAS.