Quantification of Na+, K+,
Ca2+ and Cl- ions
Ion content in leaves and roots of control and salt treated plants were
determined as described in Munns et al., (2010) with some minor
modifications. The collected samples were oven dried at 70°C for 3 days
and ground finely with motor and pestle. For ion extraction, 1 g of
finely ground sample powder was taken in 5 ml of aqua regia
(concentrated HCl: HNO3, 3:1 v/v) and boiled at 95°C for
60 min. The resulting solution was analysed by using an atomic
absorption spectrophotometer (GBC 932, Braeside, Australia). Chloride
ion concentration was determined by titrimetric method according to
Mohr’s method ( Korkmaz, 2001).