Methods
The cBioPortal for Cancer Genomics (https://www.cbioportal.org/) provides genomic and survival data from 1661 tumor-normal pairs from 1661 patients with various cancer types sequenced with MSK-IMPACT assay.6, 7 Details of tissues processing and MSK-IMPACT analysis have been described in a previous study.8 All 1661 patients have received ICIs treatment. We analyzed the number of coexisting mutations and TMB scores in 731 and 930 primary and metastatic lesions, respectively. The overall survival (OS) was calculated from the date of the first ICIs treatment to the time of recent follow-up or death. The Kaplan-Meier method was performed to obtain survival curves that were compared using the log-rank test. Subsequently, the X-tile model was used to determine the cut-off values of TMB. All statistical analyses were performed using Statistical Product and Service Solutions (SPSS) 22.0 software package. The statistical P values were two-sided, and a value ofP <0.05 was considered statistically significant.