Methods
The cBioPortal for Cancer Genomics (https://www.cbioportal.org/)
provides genomic and survival data from 1661 tumor-normal pairs from
1661 patients with various cancer types sequenced with MSK-IMPACT
assay.6, 7 Details of tissues processing and
MSK-IMPACT analysis have been described in a previous
study.8 All 1661 patients have received ICIs
treatment. We analyzed the number of coexisting mutations and TMB scores
in 731 and 930 primary and metastatic lesions, respectively. The overall
survival (OS) was calculated from the date of the first ICIs treatment
to the time of recent follow-up or death. The Kaplan-Meier method was
performed to obtain survival curves that were compared using the
log-rank test. Subsequently, the X-tile model was used to determine the
cut-off values of TMB. All statistical analyses were performed using
Statistical Product and Service Solutions (SPSS) 22.0 software package.
The statistical P values were two-sided, and a value ofP <0.05 was considered statistically significant.