Real-time PCR detection and sequence analysis of ASFV
The cycle threshold (Ct) value of ASFV detected from the blood sample
and the respective name of isolate from each pig were tabulated in Table
1. Phylogenetic tree (Fig 2) based on 399 nucleotides of partial capsid
p72 protein genome revealed that the ASFV strains from all eight cases
in this study belonged to genotype II, 100% similar to genotype II
strains reported in Africa (AY351518, AF270706), Eurasia (KJ195685,
FR682468, KM374558), China (MK33180) and Vietnam (MK554698). The
sequence data of each case was submitted to the GenBank database under
accession number from MT232743 to MT232750. Address is as follows:
http://www.ncbi.nlm.nih.gov.