Media
The semi-defined medium for the pre-culture contained 9.00 g/L glucose,
3.00 g/L KH2PO4, 4.58 g/L
K2HPO4, 0.30 g/L peptone, 0.15 g/L yeast
extract, 0.75 g/L sodium citrate dihydrate, 0.30 g/L
MgSO4 ∙ 7H2O, 0.03 g/L
CaCl2 ∙ 2H2O, 1.35 g/L
(NH4)2SO4, 1.11 g/L
NH4Cl, 50 mg/L kanamycin and 150 µL/L of a solution
containing 40.00 g/L FeSO4 ∙7H2O, 10.00 g/L MnSO4 ∙H2O, 10.00 g/L AlCl3 ∙6H2O, 7.30 g/L CoCl2 ∙6H2O, 2.00 g/L ZnSO4 ∙7H2O, 2.00 g/L NaMoO4 ∙2H2O, 1.00 g/L CuCl2 ∙2H2O, 0.50 g/L H3BO3.
For bioreactor cultivations, defined minimal media according to DeLisa,
Li, Rao, Weigand, and Bentley (1999) was used, with glucose as carbon
source. The initial glucose concentration was 20 g/L and the substrate
feed had a glucose concentration of 400 g/L.