mAb-3 (37-7-4)
The mAb-3 binds to all IgG subclass proteins (Figure 3), although the
binding to IgG1 is highly dependent on the amount of IgG subclass coated
on the beads since the IgG1 reactivity is absent for beads coated with
0.5μg per 1x106 beads of IgG subclass but high when
2μg per 1x106 beads of IgG subclass are used. At the
highest concentration tested (5 μg/mL), the reactivities against IgG1,
IgG3, and IgG4 were respectively: 1%, 95%, and 97% that of the
reactivity against IgG2 (0.5μg per 1x106 beads of IgG
subclass protein); 40%, 92%, and 99% (1μg per 1x106beads); and 78%, 93%, and 97% (2μg per 1x106beads).