Anti-human-IgG subclass-specific monoclonal antibodies
We tested 8 anti-human-IgG subclass-specific mAbs that were all PE-conjugated and purchased from the same manufacturer (SouthernBiotech, Birmingham, AL). As shown in Table 1, two mAbs were raised against human IgG1 (clone: HP6001, and 4E3), 3 against IgG2 (clone: 31-7-4, HP6002, and HP6014), 1 against IgG3 (clone: HP6050), and 2 against IgG4 (clone: HP6025, and HP6023). The mAbs were specific to the Fc, hinge, Fd (heavy chain of Fab), or pFc’ (pepsin digested Fc) regions.