mAb-2 (4E3)
Figure 2 shows that mAb-2 binds to all IgG subclass proteins, although
the cross-reactivity pattern of mAb-2 is dependent on the concentration
of the mAb and the amount of protein coated on the beads, where mAb-2
binds more weakly to IgG4 as compared to the other IgG subclass
proteins. At the highest concentration tested (5 μg/mL), the
reactivities against IgG2, IgG3, and IgG4 were respectively: 119%,
124%, and 9% that of the reactivity against IgG1 (0.5μg per
1x106 beads of IgG subclass protein); 109%, 109%,
and 20% (1μg per 1x106 beads); and 103%, 102%, and
27% (2μg per 1x106 beads).