mAb-1 (HP6001)
As illustrated in Figure 1, the titrimetric analysis of mAb-1 reveals
that mAb-1 binds to IgG1, IgG2 and IgG3 proteins, but not to IgG4. The
cross-reactivity pattern of mAb-1 is dependent on the concentration of
the mAb and the amount of protein coated on the beads, where a higher
concentration reveals a higher and wider cross-reactivity pattern. At
the highest concentration tested (5 μg/mL), the reactivities against
IgG2, IgG3, and IgG4 were respectively: 22%, 86%, and 0% that of the
reactivity against IgG1 (0.5μg per 1x106 beads of IgG
subclass protein); 46%, 68%, and 0% (1μg per 1x106beads); and 78%, 61%, and 1% (2μg per 1x106 beads).