loading page

Rapid intraoperative method for the identification of metastatic lymph nodes from thyroid carcinoma
  • +7
  • longshun shi,
  • Minhao Xie,
  • Guo Wu,
  • Jun Fan,
  • Mingming Guo,
  • Runlin Yang,
  • Jue Zhang,
  • Yi Zhang,
  • Bin Zhou,
  • Zhongwei Lv
longshun shi
NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine
Author Profile
Minhao Xie
NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine
Author Profile
Guo Wu
Hubei University of Medicine Affiliated Dongfeng Hospital
Author Profile
Jun Fan
NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine
Author Profile
Mingming Guo
NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine
Author Profile
Runlin Yang
NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine
Author Profile
Jue Zhang
Jiangsu Institution of Nuclear Medicine
Author Profile
Yi Zhang
Jiangsu Institution of Nuclear Medicine
Author Profile
Bin Zhou
Jiangsu Institution of Nuclear Medicine
Author Profile
Zhongwei Lv
Shanghai 10th People’s Hospital, Tongji University School of Medicine, Shanghai, China
Author Profile

Abstract

In this study, we established a rapid method for intraoperative identification of thyroid carcinoma metastatic lymph nodes. We developed a rapid method of thyroid globulin (Tg) detection called Tg-POCT based on time-resolved fluorescence immunoassay and validated reagent performance. Thyroid and lymph node tissues with metastatic thyroid cancer were considered as the positive group, and muscle, fiber, fat, and other tissues were considered as the negative control group to verify the above method combined with fine needle puncture. CV of the same batch number was 8.38% and that of different batch numbers was 11.24%. The minimum detection limit was 0.02 ng/mL. The test conformity of tissue samples was 100% by using the self-designed reference value. Thus, Tg-POCT is feasible as a rapid intraoperative method to identify thyroid cancer metastatic lymph nodes.