Na: number of individuals; Ho: observed heterozygosity; He: expected heterozygosity under Hardy–Weinberg equilibrium; TA°C: annealing temperature. p-values for the Hardy–Weinberg Equilibrium test, significance threshold adjusted using sequential Bonferroni correction: *p ≤ 0.05, ***p ≤ 0.001, ns = not significant .
Conclusion
Illumina MiSeq sequencing technology enables us to develop 30 pairs of microsatellites primers based on P. erinaceus populations. Seventeen among them were polymorphic in fifteen (15) West African locations and they provide the first set of microsatellite markers dedicated for P. erinaceus. These microsatellite markers will be useful for the characterization of genetic diversity and analysis of genetic structure for P. erinaceus populations.
Data Accessibility
The data have been deposited with links to BioProject accession number PRJNA604893 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)
17 Primers designed and evaluated: end of text (Table 2).
ORCID
Benziwa Nathalie Johnson ID:https://orcid.org/0000-0003-0018-8085
Conflicts of Interest
The authors have no conflicts of interest to declare.
Authors contributions
All authors contribute significantly to the present study and to the revision of the manuscript. Manuscript writing: B.N.J., with support from M.L.A.Q., G.C., L.C-K., H.V., A.D.K and K.K. DNA extractions, PCR running, DNA sequencing, microsatellites primers identification and selection: B.N.J. and H.V.
Genetic analyses and statistical treatments: B.N.J., G.C., L.C-K.
Coordination of data sampling: H.V. M.L.A.Q., K.A., A.D.K. and K.K.
Trees sampling in the field: B.N.J. and K.N.S.
Leading research project: M.L.A.Q.
Acknowledgements
This research was financially supported by: Sud Expert Plantes Développement Durable (SEP2D) program [AAP1-45], Agence Universitaire de la Francophonie (AUF) [DRAO-1448-2019-02], Programme d’Appui à la Lutte contre le Changement Climatique : PALCC –AMCC+ [DCI-ENV/2016/39289 Devis programme multi annuel n°DCI-ENV-2017/387-416], Service de Coopération et d’Action Culturelle (SCAC) and Campus France, CIRAD for the “Actions Incitatives-Soutien aux doctorants” Program, and genotyping platform of Agap unit managed by Pierre Mournet.
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