Figure legends
Fig. 1 (a-c) Rheological properties of pure GO hydrogel: (a) Complex modulus as a function of shear stress; (b) Shear stress and (c) dynamic viscosity as functions of shear rate. (d) Elastic modulus and yield stress of GO hydrogel under different Fe3+concentrations. (e) Complex modulus of pure GO and Fe3+-modified GO hydrogel. (f) Printability and (e) grid shape of GO hydrogel at different Fe3+concentrations under an optical microscope. Scale bar, 500 μm.
Fig. 2 3D printing from hydrogel to 3D contruct. (a) GO hydrogel before and after Fe3+ modification. (b) Schematic illustration of three-way porous structure. (c) Intact contructs with different filament distances. Scale bar, 2 mm.
Fig. 3 Porous structures under different freeze drying conditions. Uniform -80 °C field: (a, b) SEM images and (c) distribution of pore sizes. Uniform -20 °C field: (d, e) SEM images and (f) distribution of pore sizes. Oriented temperature field: (g-i) SEM images. Scale bar, (a, d) 1 mm; (b, e) 100 μm; (g, h) 500 μm; (i) 200 μm.
Fig. 4 Live/dead assay of HepaRG cell attachment on GO contructs. (a) Live cells, (b) dead cells, and (c) merged image. Scale bar, 200 μm.